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Structured Review

Anatrace solubilization buffer
Solubilization Buffer, supplied by Anatrace, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solubilization+buffer/buffer+solubilization/pm41813902-449-7-15
Average 86 stars, based on 1 article reviews
solubilization buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Lysis:

Article Title: Delta-type glutamate receptors are ligand-gated ion channels.
Article Snippet: This is a PDF file of a peer-reviewed paper that has been accepted for publication.. Although unedited, the content has been subjected to preliminary formatting.. Nature is providing this early version of the typeset paper as a service to our authors and readers.

Article Title: Delta-type glutamate receptors are ligand-gated ion channels
Article Snippet: .. The membranes were then resuspended in lysis buffer (20 mM Tris-HCl, pH 8.0 and 150 mM NaCl) and mechanically homogenized before solubilized in solubilization buffer (20 mM Tris-HCl, pH 8.0 and 150 mM NaCl, 1% n-dodecyl-β-d-maltopyranoside (Anatrace, D310) and 0.2% brain extract total (Avanti Research, 131101P)) for 2hrs at 4 °C with constant stirring. ..

Incubation:

Article Title: Structural basis for ligand promiscuity and high signaling activity of Kaposi's Sarcoma-associated Herpesvirus-encoded GPCR.
Article Snippet: .. The resuspended mixture was then incubated with 100mL of 2× solubilization buffer (20mM HEPES pH 8.0, 100mM NaCl, 5mM MgCl2, 5mM imidazole, 2% sodium cholate, 0.1% n-Dodecyl-β-D-Maltoside (DDM, Anatrace, D310), 4 μL calf intestinal alkaline phosphatase (CIP, NEB M0525), 5mM 2-mercaptoethanol, 10 μM GDP) under nutation for 1 h. Following solubilization, the mixture was centrifuged again at 180,000× g for 30min, and the supernatant was incubated with NiNTA resin at 4 °C for 2 hwith rotation. ..

Article Title: Inhibition mechanism of potential antituberculosis compound lansoprazole sulfide
Article Snippet: .. Isolated membranes (1.0 g) were mixed with 10 mL of solubilization buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.5 mM EDTA, 2% (w/v) GDN (Anatrace) and incubated overnight at 4 °C under stirring. ..

Protease Inhibitor:

Article Title: Structure-guided development of a potent human B 0 AT1 inhibitor effective in a mouse model of phenylketonuria
Article Snippet: .. To prepare the B 0 AT1–ACE2 complex, the harvested cells were solubilized for 2 h at 4 °C in solubilization buffer (25 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1% GDN [Anatrace], and protease inhibitor cocktail). .. After ultracentrifugation (20,000 × g , 45 min, 4 °C) and filtering through a syringe filter (0.45 μm pore size), the supernatant was incubated with M2 Anti-FLAG Affinity Resin (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.02% GDN) and incubated for 2 h at 4 °C.

Membrane:

Article Title: The dynamic basis of G-protein recognition and activation by a GPCR.
Article Snippet: G-protein-coupled receptor (GPCR) signalling occurs through heterotrimeric G proteins, whose selective activation leads to distinct cellular outcomes.. Although more than 200 GPCR–G protein complex structures have been determined, these static snapshots provide limited insight into the dynamics of G-protein association and dissociation.. Here we present cryo-electron microscopy structures of human neurotensin receptor type 1 (NTSR1) with minimally modified Go and Gq, showing how the receptor’s intracellular surface dynamically rearranges to accommodate each G-protein subtype.

Chromatography:

Article Title: The dynamic basis of G-protein recognition and activation by a GPCR.
Article Snippet: G-protein-coupled receptor (GPCR) signalling occurs through heterotrimeric G proteins, whose selective activation leads to distinct cellular outcomes.. Although more than 200 GPCR–G protein complex structures have been determined, these static snapshots provide limited insight into the dynamics of G-protein association and dissociation.. Here we present cryo-electron microscopy structures of human neurotensin receptor type 1 (NTSR1) with minimally modified Go and Gq, showing how the receptor’s intracellular surface dynamically rearranges to accommodate each G-protein subtype.

Isolation:

Article Title: Inhibition mechanism of potential antituberculosis compound lansoprazole sulfide
Article Snippet: .. Isolated membranes (1.0 g) were mixed with 10 mL of solubilization buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.5 mM EDTA, 2% (w/v) GDN (Anatrace) and incubated overnight at 4 °C under stirring. ..



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